The Investigations Division, which is commanded by Captain Delbert Fair is responsible for investigating reports of crime, apprehending suspects and taking an active role in tracking repeat offenders. The detectives are assigned either to Property or Violent Crimes. The division also gathers intelligence information, processes crime scenes and securely maintains all evidence. In addition, the division includes the Victim Assistance Program . A member of the Investigations Division also serves on the Lee County Career Criminal Task Force.

Detectives are plainclothes officers who are responsible for investigating crimes, collecting all evidence in a case, apprehending suspects and preparing the case for prosecution.